PARTIAL CHARACTERIZATION
OF PRUNUS NECROTIC RINGSPOT VIRUS ON APPLE IN EGYPT
Samah A. Mokbel
Department of Virus and Phytoplasma Research, Plant Pathology Research Institute, Agricultural Research Center., Egypt. Dr.SamahMokbel@gmail.com
Abstract
A severe isolate of Prunus necrotic ringspot
virus (PNRSV) was isolated from apple orchards in the vicinity of Nubaria
city, Beheira governorate, Egypt .
Infected-apple trees showed chlorotic, necrotic ringspots, and shoot holes on
leaves. Severely infected- trees withered, became useless, and were removed
causing severe economic losses. Reverse
transcriptase (RT) polymerase chain reaction (PCR), RT-PCR, using degenerate
primer pair for the coat protein (CP) gene of Ilarvirus amplified
products similar to those produced from peach and apricot isolates of
PNRSV-infecting stone fruits) . Dot
blotting immuno-binding assay (DBIA showed positive reaction between
PNRSV-infected apple sap and an Egyptian antiserum for PNRSV. Purified
preparation from infected leaves, using the electro-elution technique yielded
nucleoprotein which had Amax and Amin at 260 and 240 nm
respectively. Electron microscopy examination showed spherical virions with ca.
26 nm in diameter.
Key words: Prunus
necrotic ringspot virus, Ilarvirus, RT-PCR, Dot blotting immuno-binding
assay, Egypt
How to cite this article:
Abdel-Salam, A.M. and Mokbel,
A.S. (2014). Partial characterization of Prunus necrotic ringspot
virus on apple in Egypt. Egyptian J. of Virology, 11(2): 280-287.
